Faecal Inflamark (S100 A12) (F-INFL-ELISA)

Reference: 
F-INFL-ELISA

For research use only

S100A12 protein (also called Calgranulin C) is an, extracellular RAGE binding protein. It is secreted exclusively by activated granulocytes. This secretion is linked to inflammatory mechanisms. By binding to the RAGE receptor, S100A12 protein transmits a pro-inflammatory signal to endothelial and immune cells.

S100A12 protein is over-expressed at inflammatory sites and its stool concentrations are correlated with disease stage and activity. It is abundant in the bowel mucous of patients affected by inflammatory bowel disease including Crohn’s disease and ulcerative colitis.

2 helix loop helix motifs (EFhands)
Tissue-specific expression
Calcium-dependent formation of complexes
91 AA(MW 10.4 kD)

Method of measurement

Inflamark® is a sandwich ELISA. A first monoclonal antibody, immobilized on the microplate, captures the S100A12 proteins contained in the calibrators and samples. After washing, the absorbed proteins are then recognized by a second monoclonal antibody conjugated to HRP (Horse-Radish-Peroxidase). After a second incubation, the unbound reagents are eliminated by washing. Then the colorimetric reaction is started by the addition of an HRP substrate, TMB (3, 3’, 5, 5’ Tetramethyl benzidine). After the reaction is stopped by addition of sulphuric acid solution, the optical density (OD) of each well is read at 450 nm. The OD values are proportional to the S100A12 protein concentration contained in the calibrators and samples.

Analytical performances

Intra Assay CV %
N=25
Inter Assay CV % Dilution recovery % Recovery of spiked recombinant
S100 A12 %
Lower limit
of detection ng/ml
≤9.0 ≤13.0 92 to 120 95 to 102 8.95

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